Should I Heat Inactivate My Serum?

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Wed, 10 Apr 2019

Should I Heat Inactivate My Serum?

 Heat Inactivation will have a negative impact on growth

  • We feel there is generally not a good reason for doing so

  • Complement is typically not present in FBS

    • FBS will generally perform better without inactivation

    • Negative events can happen during heat inactivation

      • Potential for mistakes - Serum left too long or at wrong temperature and ruined

      • Excessive precipitation

      • “Gelling” of serum

EXAMPLE OF "GELLED" SERUM