Is Precipitation In FBS Normal?
- News & Announcements
Is the precipitation I sometimes see in my FBS normal?
Precipitation will increase in serum due to:
Precipitates do not harm cells
These precipitates occur in all serum products, including our competitors.
From Invitrogen’s/Thermo's website “GIBCO FBS is not pre-aged. Upon storage at 2 to 8°C, the possibility exists for various proteins and lipoproteins in serum (e.g., cold agglutinins, fibrinogen, vitronectin, etc.) to aggregate, and form either perceptible material or observed turbidity. This should not affect serum performance. We recommend that you store FBS at -20°C and avoid repeated freeze-thaw cycles.
From Sigma FBS Product Information Sheet: “Turbidity or flocculent material may appear upon thawing or prolonged storage at refrigerator temperature. This is a normal occurrence common to most serum products and is not detrimental to its performance”